Analytical Study of Microsomes and Isolated Subcellular Membranes from Rat Liver I. Biochemical Methods
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چکیده
The series introduced by this paper reports the results of a detailed analysis of the micro-somal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cyto
منابع مشابه
ELECTRON MICROSCOPE EXAMINATION OF SUBCELLULAR FRACTIONS III. Quantitative Analysis of the Microsomal Fraction Isolated from Rat Liver
Morphometric studies of the cellular membranes have been carried out on normal rat liver by Loud (29) and by Weibel et al . (47) . Such measurements in situ offer the advantage of allowing easy cytological identification of the structures, but they do not permit direct correlation between morphological and biochemical data on the same material . Such a correlation can be more directly establish...
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متن کاملAnalytical Study of Microsomes and Isolated Subcellular Membranes from Rat Liver
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متن کاملAnalytical study of microsomes and isolated subcellular membranes from rat liver. VI. Electron microscope examination of microsomes for cytochrome b5 by means of a ferritin-labeled antibody
The distribution of cytochrome b5 in rat liver microsomes, and in two microsomal subfractions isolated by density equilibration in a linear sucrose gradient, was studied under the electron microscope by means of a ferritin-labeled hybrid anti-cytochrome b5/anti-ferritin antibody. Results of this study show that cytochrome b5 is present in essentially all microsomal vesicles derived from endopla...
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تاریخ انتشار 1974